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KMID : 0354719920160010017
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Journal of Korean Diabetes Association
1992 Volume.16 No. 1 p.17 ~ p.23
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Increased Aldose Reductase Activity of Cultured Endothelial Cell in High Glucose Media and Reverse by Epalrestat
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나득영
우정택/김진우/김광원/김영설/최영길/서광식/팽정령
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Abstract
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Increased polyol pathway acitivity by hyperglycemia had a major role in the development of chronic diabetic complications. However the role of development in microvascular complication by increased aldose reductse activity in endothelial cell is
not
well knowen until now.
To investigate the activation of polyol pathway in endothelial cell we measured aldose reductase activity from cultured bovine pulmonary endothelial cell line cell (ATCC No CCL209). The endothelial cells were cultured in stndard media (MEM and
20%
fetal
bovine serum) or media supplemented with 100 to 500 ㎎/dl glucose. Thease studies were repeated in presense of the aldosre reductase inhibitor. Epalrestat 1 or 10nM(Ono 2235). Aldose reductase acitivities were assaed spectrophotometricaly by
recording
the decrease in NADPH absorbance under substrate of gluceraldehyde and expressed as nM/min/㎎ protein.
In supplementation of 100㎎/dl glucose the aldose reductase activity was 5.4±0.74nM/min/㎎ protein andit was increased to 15.5±2.44, 36.8±4.23, and 40.5±1.77 at 200, 300, and 500㎎/dl glucose supplementation, respectibly. The increased aldose
reductase acivity was reversed by addition of epalrestat 1 or 10nM.
These result suggest enough aldose reductase activity was existed in endothelial cell and the activity was increased to high glucose concentration. Increased polyl pathway during hyperglycemia may be involved in the pathogenesis of diabetic
complication. Aldose reductase inhibitor such as epalrestat have a role to prevent diabetic complication.
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KEYWORD
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